RABL2A Antibody (OTI4A8) - Azide and BSA Free

Western Blot: RABL2A Antibody (OTI4A8) - Azide and BSA Free [NBP2-73776] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RABL2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RABL2A.

Immunocytochemistry/Immunofluorescence: RABL2A Antibody (OTI4A8) - Azide and BSA Free [NBP2-73776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RABL2A.

Flow Cytometry: RABL2A Antibody (OTI4A8) - Azide and BSA Free [NBP2-73776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RABL2A antibody, and then analyzed by flow cytometry.

Western Blot: RABL2A Antibody (OTI4A8) - Azide and BSA Free [NBP2-73776] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RABL2A monoclonal antibody.

Flow Cytometry: RABL2A Antibody (OTI4A8) - Azide and BSA Free [NBP2-73776] - Analysis of Hela cells, using anti-RABL2A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI4A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RABL2A Antibody (OTI4A8) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human RABL2A (NP_009013) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RABL2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 25.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RABL2A Antibody (OTI4A8) - Azide and BSA Free

• FLJ78724
• MGC117180
• RAB, member of RAS oncogene family-like 2A
• rab-like protein 2A

Background

RABL2A is a member of the RAB gene family which belongs to the RAS GTPase superfamily. The proteins in the family of RAS-related signaling molecules are small GTP-binding proteins that play important roles in the regulation of exocytotic and endocytotic pathways. This gene maps to the site of an ancestral telomere fusion event and may be a subtelomeric gene. Two alternatively spliced transcript variants have been identified, both encoding the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RABL2A