Rab7a Antibody

Western Blot: Rab7a Antibody [NBP2-89146] - Analysis of RAB7 on MCF-7 cell lysate using anti-RAB7 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-89146] - Staining RAB7 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-89146] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Rab7a antibody. Counter stained with hematoxylin.

Flow Cytometry: Rab7a Antibody [NBP2-89146] - Analysis of A431 cells with RAB7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-89146] - Staining RAB7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP2-89146] - Staining RAB7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-89146] - Analysis of paraffin-embedded rat brain tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-89146] - Analysis of paraffin-embedded human liver tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-89146] - Analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP2-89146] - Analysis of paraffin-embedded mouse colon tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Product Discontinued

Rab7a Antibody Summary

• Immunogen: Recombinant protein within Human Rab7a aa 43-200 / 207. (SwissProt: P51149 Human; SwissProt: P51150 Mouse; SwissProt: P09527 Rat)
• Localization: Endosome. Lysosome.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB7A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rab7a Antibody

• CMT2B
• FLJ20819
• member RAS oncogene family
• PRO2706
• PSN
• RAB7
• Rab7a
• RAB7A, member RAS oncogene family
• Ras-associated protein RAB7
• ras-related protein Rab-7a

Background

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB7A