Rab7a Antibody (SN202-03)

Western Blot: Rab7a Antibody (SN202-03) [NBP2-67732] - Western blot analysis of Rab7a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: Rab7a Antibody (SN202-03) [NBP2-67732] - Staining RAB7 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rab7a Antibody (SN202-03) [NBP2-67732] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Flow Cytometry: Rab7a Antibody (SN202-03) [NBP2-67732] - Analysis of K562 cells with RAB7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Rab7a Antibody (SN202-03) [NBP2-67732] - Analysis of RAB7 on different lysates using anti-RAB7 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: C2C12

Immunocytochemistry/Immunofluorescence: Rab7a Antibody (SN202-03) [NBP2-67732] - Staining RAB7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Rab7a Antibody (SN202-03) [NBP2-67732] - Staining RAB7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Rab7a Antibody (SN202-03) [NBP2-67732] - Analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rab7a Antibody (SN202-03) [NBP2-67732] - Analysis of paraffin-embedded human kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Rab7a Antibody (SN202-03) [NBP2-67732] - Analysis of paraffin-embedded mouse kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.

Product Discontinued

Rab7a Antibody (SN202-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Rab7a aa 158-207 / 207. (SwissProt: P51149 Human; SwissProt: P51150 Mouse; SwissProt: P09527 Rat)
• Localization: Cytoplasmic vesicle, Lysosome membrane, Melanosome membrane, Lipid droplet.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAB7A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rab7a Antibody (SN202-03)

• CMT2B
• FLJ20819
• member RAS oncogene family
• PRO2706
• PSN
• RAB7
• Rab7a
• RAB7A, member RAS oncogene family
• Ras-associated protein RAB7
• ras-related protein Rab-7a

Background

RAB7 is a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB7A