RAB3B Antibody (3F12)

Western Blot: RAB3B Antibody (3F12) [H00005865-M01] - Analysis of RAB3B expression in transfected 293T cell line by RAB3B monoclonal antibody (M01), clone 3F12.Lane 1: RAB3B transfected lysate(24.758 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: RAB3B Antibody (3F12) [H00005865-M01] - Analysis of monoclonal antibody to RAB3B on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: RAB3B Antibody (3F12) [H00005865-M01] - Immunoperoxidase of monoclonal antibody to RAB3B on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

Western Blot: RAB3B Antibody (3F12) [H00005865-M01] - RAB3B monoclonal antibody (M01), clone 3F12. Analysis of RAB3B expression in IMR-32.

ELISA: RAB3B Antibody (3F12) [H00005865-M01] - Detection limit for recombinant GST tagged RAB3B is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 3F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RAB3B Antibody (3F12) Summary

• Immunogen: RAB3B (AAH05035, 120 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IKTYSWDNAQVILVGNKCDMEEERVVPTEKGQLLAEQLGFDFFEASAKENISVRQAFERLVDAICDKMSDSLDTDPSMLGSSKNTRLSDTPPLLQQNCSC
• Specificity: RAB3B - RAB3B, member RAS oncogene family
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB3B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAB3B Antibody (3F12)

• brain antigen RAB3B
• RAB3B, member RAS oncogene family
• ras-related protein Rab-3B

Background

Protein transport. Probably involved in vesicular traffic

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAB3B Antibody (H00005865-M01)(3)

Publications using H00005865-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Roberto C, Valeria R, Cristina N et al. Gut-derived endotoxin stimulates factor viii secretion from endothelial cells. implications for hypercoagulability in cirrhosis. J Hepatol 2017-07-14 [PMID: 28716745]
• Vogel GF, Klee KM, Janecke AR et al. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol. 2015-11-09 [PMID: 26553929]

Bioinformatics

• Gene Symbol: RAB3B
• Entrez:
  • 5865(Human)
• OMIM:
  • 179510(Human)
• Uniprot:
  • AAH05035(Human)