QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin] [NBP2-26649APC] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review/

• Reactivity: Hu, Mu
• Applications: WB, Flow, IP, B/N
• Clone: 6A8.6F10.1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin] Summary

• Immunogen: Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells.The antibody reacts with the Qa-1b Class Ib molecule.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: QA1b
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation
  • Neutralization
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for QA1b Antibody (6A8.6F10.1A6) [Allophycocyanin]

• H2-Q1
• H2-T23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for QA1b Antibody (NBP2-26649APC) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 02/21/2019

Summary

• Application: Flow Cytometry
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: AB021909A-102416-APC

Comments

• Comments: Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue histogram), and in QA1b-stained cells (red histogram).

Bioinformatics

• Gene Symbol: QA1b