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![Western Blot: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in mouse thymus tissue lysate using NBP2-26649 at 2 ug/mL.](http://images.novusbio.com/fullsize/QA1b-Antibody-6A8-6F10-1A6-Western-Blot-NBP2-26649-img0004.jpg "Western Blot: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in mouse thymus tissue lysate using NBP2-26649 at 2 ug/mL.")
![Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. CNS cells of EAE mice stained for viable cells using APC conjugated QA1B antibody (NBP2-26649APC). Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review.](http://images.novusbio.com/fullsize/QA1b-Antibody-6A8-6F10-1A6-Flow-Cytometry-NBP2-26649-img0008.jpg)
![Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells.](http://images.novusbio.com/fullsize/QA1b-Antibody-6A8-6F10-1A6-Flow-Cytometry-NBP2-26649-img0003.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis using the Biotin conjugate of NBP2-26649. Staining of resting and ConA activated (5 ug/mL for 72 hr) BALB/C splenocytes using 0.5 ug of Qa-1b biotinylated antibody. CD3 positive cells were gated. Streptavidin - PE (SA-PE) was used as secondary antibody. a) SA-PE resting cells. b) SA-PE ConA activated cells. c) Qa-1b resting cells. d) Qa-1b ConA activated cells.](http://images.novusbio.com/fullsize/QA1b-Antibody-6A8-6F10-1A6-Flow-Cytometry-NBP2-26649-img0006.jpg)
![Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of Qa-1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in the entire population of cells Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/QA1b-Antibody-6A8-6F10-1A6-Flow-Cytometry-NBP2-26649-img0007.jpg)