QA1b Antibody (6A8.6F10.1A6) - BSA Free

Western Blot: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in mouse thymus tissue lysate using NBP2-26649 at 2 ug/mL.

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for QA1b, CD8, CD4, TNF, IFNg, IL-17, plus for viability to exclude dead cells. CNS cells of EAE mice stained for viable cells using APC conjugated QA1B antibody (NBP2-26649APC). Viable, CD8+ cells were gated and QA1b staining is showed in control cells, non-stained for QA1b (blue), and in QA1b-stained cells (red). Image from verified customer review.

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells.

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis using the Biotin conjugate of NBP2-26649. Staining of resting and ConA activated (5 ug/mL for 72 hr) BALB/C splenocytes using 0.5 ug of Qa-1b biotinylated antibody. CD3 positive cells were gated. Streptavidin - PE (SA-PE) was used as secondary antibody. a) SA-PE resting cells. b) SA-PE ConA activated cells. c) Qa-1b resting cells. d) Qa-1b ConA activated cells.

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of Qa-1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in the entire population of cells Image using the Azide Free form of this antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IP, B/N
• Clone: 6A8.6F10.1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

QA1b Antibody (6A8.6F10.1A6) - BSA Free Summary

• Immunogen: Animals were immunized with a synthetic peptide from the alpha-2 domain of Qa-1b. Qa-1 is a Class Ib molecule recognized by cytotoxic T cells and natural killer cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the Qa-1b Class Ib molecule.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: QA1b
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation
  • Neutralization
  • Western Blot 1 - 2 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for QA1b Antibody (6A8.6F10.1A6) - BSA Free

• H2-Q1
• H2-T23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for QA1b Antibody (NBP2-26649)(1)

Publication using NBP2-26649

• Brownlie D, Doughty-Shenton D, Yh Soong D, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor beta Journal for immunotherapy of cancer 2021-01-01 [PMID: 33472858] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: QA1b
• Uniprot:
  • NP_001129540()