PTK7/CCK4 Antibody (OTI2E7)

Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CCK4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CCK4.

Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CCK4 antibody, and then analyzed by flow cytometry.

Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCK4.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF
• Clone: OTI2E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PTK7/CCK4 Antibody (OTI2E7) Summary

• Description: Novus Biologicals Mouse PTK7/CCK4 Antibody (OTI2E7) (NBP2-01486) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTK7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:200-500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTK7/CCK4 Antibody (OTI2E7)

• CCK4
• CCK-4
• CCK4Colon carcinoma kinase 4
• colon carcinoma kinase-4
• Protein-tyrosine kinase 7
• Pseudo tyrosine kinase receptor 7
• PTK7 protein tyrosine kinase 7
• PTK7
• Tyrosine-protein kinase-like 7

Background

Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTK7
• Entrez:
  • 5754(Human)