PTF1A Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PTF1A at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PTF1A was detected in immersion fixed human pancreatic cancer cells using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PTF1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

PTF1A Antibody Summary

• Immunogen: E. coli-derived recombinant human PTF1A
  Met1-Pro92
  Accession # Q7RTS3
• Specificity: Detects human PTF1A in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTF1A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTF1A Antibody

• bHLH transcription factor p48
• BHLHA29
• bHLHa29exocrine pancreas-specific transcription factor p48
• Class A basic helix-loop-helix protein 29
• p48 DNA-binding subunit of transcription factor PTF1
• pancreas specific transcription factor, 1a
• pancreas transcription factor 1 subunit alpha
• Pancreas-specific transcription factor 1a
• PTF1A
• PTF1P48
• PTF1-p48
• PTF1-p48class II bHLH protein PTF1A

Background

PTF1A (Pancreas transcription factor 1a; also p48 and bHLHA29) is a cell specific member of the bHLH family of proteins. Although its predicted MW is 41 kDa, a high ratio of Pro, Leu and Ala increases its MW to 48 kDa in SDS-PAGE. PTF1A forms a trimeric transcription factor complex with a p75 subunit (HEB, E2 or E47) and a p64 subunit (usually RBP-L). Within this complex, unfortunately termed PTF1, p48/PTF1A binds to a DNA E-box while p64 binds to a TC-box. This regulates the gene transcription of about 20 enzymes synthesized by pancreatic acinar cells. Human PTF1A is 328 amino acids (aa) in length. It contains one basic DNA binding HLH motif (aa 150-175) and a dimerization interface (aa 178-219). Over aa 1-92, human PTF1A shares 88% aa identity with mouse PTF1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTF1A Antibody (AF6119)(1)

Publication using AF6119

• Bernard Pessac, Mehdi Ait Bara, David Ford, Gopi K. Patibandla, David Trisler Hematopoietic progenitors express embryonic stem cell and germ layer genes Comptes Rendus Biologies 2011-04-01 [PMID: 21513899] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PTF1A
• Uniprot:
  • Q7RTS3(Human)