PSMD11 Antibody (1F4) - BSA Free

Western Blot: PSMD11 Antibody (1F4) [NBP1-30252] - The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu, Mu, Ce
• Applications: WB, ELISA
• Clone: 1F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PSMD11 Antibody (1F4) - BSA Free Summary

• Description: Novus Biologicals Mouse PSMD11 Antibody (1F4) - BSA Free (NBP1-30252) is a monoclonal antibody validated for use in WB and ELISA. Anti-PSMD11 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PSMD11 (1-422aa) purified from E. coli (NP_002806).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMD11
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:250-1:1000
• Theoretical MW: 47.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 22922647). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PSMD11 Antibody (1F4) - BSA Free

• MGC3844,26S proteasome regulatory subunit RPN6
• p44.5,26S proteasome regulatory subunit 9
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5
• Rpn6,26S proteasome regulatory subunit S9
• S9,26S proteasome non-ATPase regulatory subunit 11

Background

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMD11 Antibody (NBP1-30252)(3)

Publications using NBP1-30252

• Vilchez D, Boyer L, Morantte I et al. Increased proteasome activity in human embryonic stem cells is regulated by PSMD11 Nature 2012-09-13 [PMID: 22972301] (WB, Human)
• Vilchez D, Morantte I, Liu Z et al. RPN-6 determines C. elegans longevity under proteotoxic stress conditions Nature 2012-09-13 [PMID: 22922647] (WB, C. elegans)
• Liu Z, Lee J, Krummey S et al. The kinase LRRK2 is a regulator of the transcription factor NFAT that modulates the severity of inflammatory bowel disease. Nat Immunol;12(11):1063-1070. 2011-01-01 [PMID: 21983832]

Bioinformatics

• Gene Symbol: PSMD11
• Entrez:
  • 5717(Human)
• Uniprot:
  • O00231(Human)