PSMD11 Antibody (1F4)

Images

 
Western Blot: PSMD11 Antibody (AT1F4) [NBP1-30252] - The extracts of mouse kidney (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMD11 (1:250). Proteins were visualized using a ...read more

Product Details

Summary
Reactivity Hu, Mu, CeSpecies Glossary
Applications WB, ELISA
Clone
1F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PSMD11 Antibody (1F4) Summary

Immunogen
Recombinant human PSMD11 (1-422aa) purified from E. coli (NP_002806).
Specificity
This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PSMD11 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PSMD11
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1000
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250-1:1000.
Theoretical MW
47.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-30252 in the following applications:

  • WB
    2 publications

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 22922647). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PSMD11 Antibody (1F4)

  • MGC3844,26S proteasome regulatory subunit RPN6
  • p44.5,26S proteasome regulatory subunit 9
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5
  • Rpn6,26S proteasome regulatory subunit S9
  • S9,26S proteasome non-ATPase regulatory subunit 11

Background

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMD11 Antibody (NBP1-30252)(3)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMD11
Entrez
Uniprot