PSMB5 Antibody - BSA Free

Immunohistochemistry;PSMB5 Antibody[NBP3-29771]Detection of human PSMB5 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PSMB5 used at a dilution of 1:5,000 . Detection: DAB

Immunoprecipitation:PSMB5 AntibodyNBP3-29772] -Detection of human PSMB5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMB5 antibody NBP3-29772 (lot NBP3-29772-1) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated PSMB5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSMB5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PSMB5 Antibody - BSA Free (NBP3-29772) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 213 and 263
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMB5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for PSMB5 Antibody - BSA Free

• LMPX
• LMPXproteasome beta 5 subunit
• Macropain epsilon chain
• MB1
• MB1EC 3.4.25.1
• Multicatalytic endopeptidase complex epsilon chain
• proteasome (prosome, macropain) subunit, beta type, 5
• proteasome catalytic subunit 3
• Proteasome chain 6
• Proteasome epsilon chain
• proteasome subunit beta type-5
• Proteasome subunit MB1
• Proteasome subunit X
• proteasome subunit, beta type, 5
• PSMB5
• PSX large multifunctional protease X
• XMGC104214

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMB5