PSMB5 Antibody - Azide and BSA Free

Western Blot: PSMB5 Antibody [H00005693-D01P] - Analysis of PSMB5 expression in mouse spleen.

Western Blot: PSMB5 Antibody [H00005693-D01P] - Analysis of PSMB5 expression in transfected 293T cell line by PSMB5 polyclonal antibody.Lane 1: PSMB5 transfected lysate(28.50 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PSMB5 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PSMB5 (NP_002788.1, 1 a.a. - 263 a.a.) full-length human protein. MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP
• Specificity: Reacts with proteasome (prosome, macropain) subunit, beta type, 5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMB5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PSMB5 Antibody - Azide and BSA Free

• LMPX
• LMPXproteasome beta 5 subunit
• Macropain epsilon chain
• MB1
• MB1EC 3.4.25.1
• Multicatalytic endopeptidase complex epsilon chain
• proteasome (prosome, macropain) subunit, beta type, 5
• proteasome catalytic subunit 3
• Proteasome chain 6
• Proteasome epsilon chain
• proteasome subunit beta type-5
• Proteasome subunit MB1
• Proteasome subunit X
• proteasome subunit, beta type, 5
• PSMB5
• PSX large multifunctional protease X
• XMGC104214

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMB5 Antibody (H00005693-D01P)(1)

Publication using H00005693-D01P

• Grimm S, Ott C, Horlacher M et al. Advanced glycation end products-induced formation of immunoproteasomes: involvement of the receptor for AGEs and Jak2/STAT1. Biochem J. 2012-08-15 [PMID: 22892029]

Bioinformatics

• Gene Symbol: PSMB5
• Entrez:
  • 5693(Human)
• Uniprot:
  • NP_002788.1(Human)