Recombinant Human PSMA4 His Protein Summary
| Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-261 of Human PSMA4 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSRRYD SRTTIFSPEG RLYQVEYAME AIGHAGTCLG ILANDGVLLA AERRNIHKLL DEVFFSEKIY KLNEDMACSV AGITSDANVL TNELRLIAQR YLLQYQEPIP CEQLVTALCD IKQAYTQFGG KRPFGVSLLY IGWDKHYGFQ LYQSDPSGNY GGWKATCIGN NSAAAVSMLK QDYKEGEMTL KSALALAIKV LNKTMDVSKL SAEKVEIATL TRENGKTVIR VLKQKEVEQL IKKHEEEEAK AEREKKEKEQ KEKDK |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
PSMA4 |
| Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 0.1 mM PMSF |
| Preservative |
No Preservative |
| Concentration |
1 mg/ml |
| Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human PSMA4 His Protein
Background
PSMA4, also known as proteasome subunit alpha type 4, is a member of the peptidase T1A family, that is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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