PSMA4 Overexpression Lysate (Native)

Images

 
Western Blot: PSMA4 Overexpression Lysate (Adult Normal) [NBL1-14873] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSMA4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PSMA4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202786 and based on accession number NM_002789. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 4 (PSMA4), transcript variant 1, mRNA.
Gene
PSMA4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PSMA4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PSMA4 Overexpression Lysate (Native)

  • HC9EC 3.4.25.1
  • HsT17706
  • Macropain subunit C9
  • MGC12467
  • MGC24813
  • Multicatalytic endopeptidase complex subunit C9
  • proteasome (prosome, macropain) subunit, alpha type, 4
  • Proteasome component C9
  • proteasome subunit alpha type-4
  • proteasome subunit HC9
  • Proteasome subunit L
  • PSC9MGC111191

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PSMA4 Lysate (NBL1-14873) (0)

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Bioinformatics

Gene Symbol PSMA4