PSD93 Antibody (JM41-04)

Western Blot: PSD93 Antibody (JM41-04) [NBP2-75631] - Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate

Flow Cytometry: PSD93 Antibody (JM41-04) [NBP2-75631] - Analysis of SH-SY5Y cells with PSD93 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: PSD93 Antibody (JM41-04) [NBP2-75631] - Analysis of PSD93 on different lysates using anti-PSD93 antibody at 1/1,000 dilution.Positive control:Lane 1: Hela Lane 2: Mouse ThymusLane 3: SH-SY5Y

Product Discontinued

PSD93 Antibody (JM41-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human PSD93 aa 600-660. (SwissProt: Q15700 Human; SwissProt: Q91XM9 Mouse; SwissProt: Q63622 Rat)
• Localization: Cell junction, Cell membrane, Cell projection, Membrane, Synapse.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DLG2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PSD93 Antibody (JM41-04)

• Chapsyn-110
• discs, large homolog 2 (Drosophila)
• discs, large homolog 2, chapsyn-110 (Drosophila)
• discs, large homolog 2, chapsyn-110
• disks large homolog 2
• DKFZp781D1854
• DKFZp781E0954
• FLJ37266
• MGC131811
• Postsynaptic density protein PSD-93
• PSD-93
• PSD93,110kDa

Background

Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG2