PRPF8 Antibody - BSA Free

Western Blot: PRPF8 Antibody [NBP2-22272] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: PRPF8 Antibody [NBP2-22272] - Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- PRPF8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PRPF8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PRPF8 Antibody - BSA Free (NBP2-22272) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 850 and 900 of human Pre-mRNA-Processing Factor 8 using the numbering given in entry NP_006436.3 (GeneID 10594).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRPF8 Antibody - BSA Free

• apoptosis-regulated protein 1
• apoptosis-regulated protein 2
• p220
• precursor mRNA processing protein
• pre-mRNA-processing-splicing factor 8
• PRP8 homolog
• PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• PRP8 pre-mRNA processing factor 8 homolog (yeast)
• Prp8
• PRP8,220 kDa U5 snRNP-specific protein
• PRPC8HPRP8
• RP13
• Splicing factor Prp8
• U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF8
• Uniprot:
  • NP_006436.3(Human)