PRPF8 Antibody (S06-1I7)

Western Blot: PRPF8 Antibody (S06-1I7) [NBP3-19748] - Western blot detection of PRPF8 in Jurkat, Raji lysates using PRPF8 antibody. Predicted band size: 274kDa. Observed band size: 274kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S06-1I7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PRPF8 Antibody (S06-1I7) Summary

• Description: Novus Biologicals Rabbit PRPF8 Antibody (S06-1I7) (NBP3-19748) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human PRPF8 (Uniprot # Q6P2Q9)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRPF8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 274 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PRPF8 Antibody (S06-1I7)

• apoptosis-regulated protein 1
• apoptosis-regulated protein 2
• p220
• precursor mRNA processing protein
• pre-mRNA-processing-splicing factor 8
• PRP8 homolog
• PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• PRP8 pre-mRNA processing factor 8 homolog (yeast)
• Prp8
• PRP8,220 kDa U5 snRNP-specific protein
• PRPC8HPRP8
• RP13
• Splicing factor Prp8
• U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF8