Protocadherin-8 Antibody

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Protocadherin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin-8 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Protocadherin-8 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
  Lys30-Pro749
  Accession # O95206
• Specificity: Detects human Protocadherin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Protocadherin-10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCDH8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin-8 Antibody

• Arcadlin
• PAPC
• PCDH8
• protocadherin 8
• Protocadherin8
• Protocadherin-8

Background

Protocadherin 8 (PCDH-8, Arcadlin) is a 110-115 kDa group F member of the protocadherin family of molecules. It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca⁺⁺-dependent homophilic complexes. In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP. Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length. It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region. There are six cadherin domains in the ECD (aa 30-723). Multiple splice variants exist. Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527. Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDH8
• Uniprot:
  • O95206()