Protocadherin-8 Antibody

Images

 
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Protocadherin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) followed by ...read more
Protocadherin‑8 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human Protocadherin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Protocadherin-8 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
Lys30-Pro749
Accession # O95206
Specificity
Detects human Protocadherin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Protocadherin-10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PCDH8
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin-8 Antibody

  • Arcadlin
  • PAPC
  • PCDH8
  • protocadherin 8
  • Protocadherin8
  • Protocadherin-8

Background

Protocadherin 8 (PCDH-8, Arcadlin) is a 110-115 kDa group F member of the protocadherin family of molecules. It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca++-dependent homophilic complexes. In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP. Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length. It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region. There are six cadherin domains in the ECD (aa 30-723). Multiple splice variants exist. Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527. Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

6510-CA
Species: Hu
Applications: BA
NBP2-52979
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00005097-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-05950
Species: Hu, Mu
Applications: ICC/IF, WB
AF7669
Species: Hu, Mu, Rt
Applications: WB
AF198
Species: Mu
Applications: IHC, WB
NBP2-61772
Species: Hu
Applications: ELISA, Flow, ICC/IF, WB
210-TA
Species: Hu
Applications: BA
MAB8626
Species: Hu
Applications: CyTOF-ready, Flow
H00027328-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NBP1-87308
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-46340
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00083259-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-77005
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
5604-CA
Species: Hu
Applications: BA

Publications for Protocadherin-8 Antibody (AF5434) (0)

There are no publications for Protocadherin-8 Antibody (AF5434).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protocadherin-8 Antibody (AF5434) (0)

There are no reviews for Protocadherin-8 Antibody (AF5434). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Protocadherin-8 Antibody (AF5434) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Protocadherin-8 Products

Bioinformatics Tool for Protocadherin-8 Antibody (AF5434)

Discover related pathways, diseases and genes to Protocadherin-8 Antibody (AF5434). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Protocadherin-8 Antibody (AF5434)

Discover more about diseases related to Protocadherin-8 Antibody (AF5434).
 

Pathways for Protocadherin-8 Antibody (AF5434)

View related products by pathway.

PTMs for Protocadherin-8 Antibody (AF5434)

Learn more about PTMs related to Protocadherin-8 Antibody (AF5434).

Blogs on Protocadherin-8

There are no specific blogs for Protocadherin-8, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Protocadherin-8 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PCDH8
Uniprot