Protocadherin-8 Antibody (562615)

Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Monoclonal Antibody (Catalog # MAB5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 562615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Protocadherin-8 Antibody (562615) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
  Lys30-Thr479
  Accession # O95206
• Specificity: Detects human Protocadherin-8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protocadherin-1, -10, or -15 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCDH8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin-8 Antibody (562615)

• Arcadlin
• PAPC
• PCDH8
• protocadherin 8
• Protocadherin8
• Protocadherin-8

Background

Protocadherin 8 (PCDH-8) is a 160 kDa group F member of the protocadherin family of molecules.  It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca⁺⁺-dependent homophilic complexes.  In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP.  Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length.  It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region.  There are six cadherin domains in the ECD (aa 30-723).  Multiple splice variants exist.  Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527.  Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protocadherin-8 Antibody (MAB5434)(1)

Publication using MAB5434

• Maddox , Jessica, Shakya , Arvind, South , Samuel, Shelton , Dawne, Andersen , Jared N, Chidester , Stephani, Kang , Jinsuk, Gligorich , Keith M, Jones , David A, Spangrude , Gerald J, Welm , Bryan E, Tantin , Dean Transcription factor Oct1 is a somatic and cancer stem cell determinant. PLoS Genet, 2012-11-08;8(11):e1003048. 11/8/2012 [PMID: 23144633]

Bioinformatics

• Gene Symbol: PCDH8
• Uniprot:
  • O95206()