Protocadherin-8 Antibody (562615) [Alexa Fluor® 488]

• Applications: ICC/IF
• Clone: 562615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Protocadherin-8 Antibody (562615) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
  Lys30-Thr479
  Accession # O95206
• Specificity: Detects human Protocadherin-8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protocadherin-1, -10, or -15 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Protocadherin 8 (PCDH-8) is a 160 kDa group F member of the protocadherin family of molecules.  It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca⁺⁺-dependent homophilic complexes.  In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP.  Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length.  It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region.  There are six cadherin domains in the ECD (aa 30-723).  Multiple splice variants exist.  Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527.  Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.