Protein O-Glucosyltransferase 1/POGLUT1/KTELC1 Antibody (737512)

Protein O-Glucosyltransferase 1/POGLUT was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Protein O-Glucosyltransferase 1/POGLUT Monoclonal Antibody (Catalog # MAB64371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 737512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Protein O-Glucosyltransferase 1/POGLUT1/KTELC1 Antibody (737512) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Protein O‑Glucosyltransferase 1/POGLUT
  Arg24-Leu388
  Accession # Q8NBL1
• Specificity: Detects human Protein O‑Glucosyltransferase 1/POGLUT in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: POGLUT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein O-Glucosyltransferase 1/POGLUT1/KTELC1 Antibody (737512)

• 9630046K23Rik
• C3orf9
• CAP10-like protein, 46 kDa
• chromosome 3 open reading frame 9
• CLP46
• EC 2.4.1.-
• hCLP46CAP10-like 46 kDa protein
• KDELC family like 1
• KDELCL1
• KTEL (Lys-Tyr-Glu-Leu) containing 1
• KTEL motif-containing protein 1
• KTELC1
• MDS010
• MDSRP
• MDSRPKTELC1
• MGC32995
• Myelodysplastic syndromes relative protein
• POGLUT1
• protein O-glucosyltransferase 1
• Rumi
• x 010 protein

Background

Protein O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). POGLUT1 has hydrolase activity on UDP-Glc in a phosphatase-coupled glycosyltransferase assay (4).

1. Acar, M. et al. (2008) Cell 132:247.
2. Teng, Y. et al. (2006) Gene 371:7
3. Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
4. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POGLUT1
• Uniprot:
  • Q8NBL1()