Protein Kinase D2 Antibody (JU08-33)

Western Blot: Protein Kinase D2 Antibody (JU08-33) [NBP2-75629] - Analysis of Protein Kinase D2 on A549 cell using anti-Protein Kinase D2 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody (JU08-33) [NBP2-75629] - Staining Protein Kinase D2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (JU08-33) [NBP2-75629] - Analysis of paraffin-embedded human tonsil tissue using anti-Protein Kinase D2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Protein Kinase D2 Antibody (JU08-33) [NBP2-75629] - Analysis of SH-SY-5Y cells with Protein Kinase D2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody (JU08-33) [NBP2-75629] - Staining Protein Kinase D2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Protein Kinase D2 Antibody (JU08-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Protein Kinase D2 aa 181-306 / 878. (SwissProt: Q9BZL6 Human)
• Localization: Membrane. Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Protein Kinase D2 Antibody (JU08-33)

• DKFZP586E0820
• EC 2.7.11
• EC 2.7.11.13
• nPKC-D2
• PKD2HSPC187
• PRKD2
• protein kinase D2
• serine/threonine-protein kinase D2

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKD2