Proteasome 20S beta 3 Overexpression Lysate (Native)

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Western Blot: Proteasome 20S beta 3 Overexpression Lysate (Adult Normal) [NBL1-14878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proteasome 20S beta 3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Proteasome 20S beta 3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201296 and based on accession number NM_002795. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens proteasome (prosome, macropain) subunit, beta type, 3 (PSMB3), mRNA.
Gene
PSMB3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Proteasome 20S beta 3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Proteasome 20S beta 3 Overexpression Lysate (Native)

  • EC 3.4.25.1
  • HC10-II
  • MGC4147
  • proteasome (prosome, macropain) subunit, beta type, 3
  • Proteasome chain 13
  • Proteasome component C10-II
  • proteasome subunit beta type-3
  • Proteasome theta chain

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Proteasome 20S beta 3 Lysate (NBL1-14878) (0)

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Bioinformatics

Gene Symbol PSMB3