![Western Blot: Proprotein Convertase 2/PCSK2 Antibody [H00005126-D01P] - Analysis of PCSK2 expression in mouse liver.](http://images.novusbio.com/fullsize2/Proprotein-Convertase-2-PCSK2-Antibody-Western-Blot-H00005126-D01P-img0005.jpg "Western Blot: Proprotein Convertase 2/PCSK2 Antibody [H00005126-D01P] - Analysis of PCSK2 expression in mouse liver.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | PCSK2 (NP_002585.2, 1 a.a. - 638 a.a.) full-length human protein. MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKQQLERDPRVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN |
| Specificity | PCSK2 - proprotein convertase subtilisin/kexin type 2, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PCSK2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Immunocytochemistry-Immunofluorescence-H00005122-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Immunohistochemistry-Paraffin-H00005122-M02-img0012.jpg)
![Sandwich ELISA: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Detection limit for recombinant GST tagged PCSK1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Sandwich-ELISA-H00005122-M02-img0013.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/images2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.](http://images.novusbio.com/images2/POMC-Antibody-Immunohistochemistry-NB100-1533-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cbx8 Antibody [NBP1-83224] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/Cbx8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83224-img0008.jpg)
![Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/Cbx8-Antibody-Immunohistochemistry-Paraffin-NBP1-83224-img0009.jpg)
![Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/Cbx8-Antibody-Immunohistochemistry-Paraffin-NBP1-83224-img0010.jpg)
![Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/ACTR1B-Antibody-Western-Blot-NBP2-15275-img0001.jpg)
![Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/ACTR1B-Antibody-Western-Blot-NBP2-15275-img0002.jpg)
![Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-15275] - Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.](http://images.novusbio.com/images2/ACTR1B-Antibody-Immunohistochemistry-Paraffin-NBP2-15275-img0003.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/BTG1-Antibody-(4C10)-Immunohistochemistry-NBP2-46587-img0001.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/BTG1-Antibody-(4C10)-Immunohistochemistry-NBP2-46587-img0002.jpg)
![Immunohistochemistry: BTG1 Antibody (4C10) [NBP2-46587] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/BTG1-Antibody-(4C10)-Immunohistochemistry-NBP2-46587-img0003.jpg)
![Western Blot: Polycystin 2 Antibody [NBP3-06363] - Anti-PKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDa](http://images.novusbio.com/images2/Polycystin-2-Antibody-Western-Blot-NBP3-06363-img0001.jpg)
![Immunoprecipitation: Polycystin 2 Antibody [NBP3-06363] - PKD2 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PKD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-PKD2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size: 80 kDa](http://images.novusbio.com/images2/Polycystin-2-Antibody-Immunoprecipitation-NBP3-06363-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody [NBP3-06363] - Immunofluorescence staining of PKD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PKD2 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06363-img0003.jpg)
![Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections. Image from verified customer review.](http://images.novusbio.com/images2/Carboxypeptidase-E-CPE-Antibody-Immunohistochemistry-Frozen-NBP2-15699-img0008.jpg)
![Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/images2/Carboxypeptidase-E-CPE-Antibody-Western-Blot-NBP2-15699-img0007.jpg)
![Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/images2/Carboxypeptidase-E-CPE-Antibody-Western-Blot-NBP2-15699-img0006.jpg)
![Immunohistochemistry: Proprotein convertase PC4 Antibody [NBP1-88010] - Staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.](http://images.novusbio.com/images2/Proprotein-convertase-PC4-Antibody-Immunohistochemistry-NBP1-88010-img0003.jpg)
![Immunohistochemistry-Paraffin: Proprotein convertase PC4 Antibody [NBP1-88010] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/images2/Proprotein-convertase-PC4-Antibody-Immunohistochemistry-Paraffin-NBP1-88010-img0004.jpg)
![Immunohistochemistry-Paraffin: IFRD1 Antibody [NBP2-24679] - Immunohistochemical analysis of IFRD1 in formalin-fixed, paraffin-embedded humanrectum tissue using an isotype control (top) and NBP2-24679 (bottom) at 5 ug/ml.](http://images.novusbio.com/images2/IFRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-24679-img0001.jpg)
![Western Blot: IFRD1 Antibody [NBP2-24679] - Analysis of Tis7 in HeLa cell lysate in the 1) absence 2) presence of immunizing peptide and 3) RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 4.0 ug/ml respectively.](http://images.novusbio.com/images2/IFRD1-Antibody-Western-Blot-NBP2-24679-img0002.jpg)
![Flow Cytometry: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PCSK7 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/Proprotein-Convertase-7-PCSK7-Antibody-(OTI1B8)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-73621-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Proprotein Convertase 7/PCSK7 Antibody (OTI1B8) - Azide and BSA Free [NBP2-73621] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PCSK7.](http://images.novusbio.com/images2/Proprotein-Convertase-7-PCSK7-Antibody-(OTI1B8)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73621-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Proprotein Convertase 2/PCSK2 Antibody (H00005126-D01P)Discover more about diseases related to Proprotein Convertase 2/PCSK2 Antibody (H00005126-D01P).
| Pathways for Proprotein Convertase 2/PCSK2 Antibody (H00005126-D01P)View related products by pathway.
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| Gene Symbol | PCSK2 |
| Entrez |
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| Uniprot |
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![Western Blot: Proprotein Convertase 2/PCSK2 Antibody [H00005126-D01P] - Analysis of PCSK2 expression in human liver.](http://images.novusbio.com/fullsize2/Proprotein-Convertase-2-PCSK2-Antibody-Western-Blot-H00005126-D01P-img0004.jpg "Western Blot: Proprotein Convertase 2/PCSK2 Antibody [H00005126-D01P] - Analysis of PCSK2 expression in human liver.")
![Western Blot: Proprotein Convertase 2/PCSK2 Antibody [H00005126-D01P] - Analysis of PCSK2 expression in transfected 293T cell line by PCSK2 polyclonal antibody.Lane 1: PCSK2 transfected lysate(70.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/Proprotein-Convertase-2-PCSK2-Antibody-Western-Blot-H00005126-D01P-img0006.jpg "Western Blot: Proprotein Convertase 2/PCSK2 Antibody [H00005126-D01P] - Analysis of PCSK2 expression in transfected 293T cell line by PCSK2 polyclonal antibody.Lane 1: PCSK2 transfected lysate(70.60 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Thyroid Gland at dilution of 8ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0002.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PC6 Antibody [NBP1-87326] - Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.](http://images.novusbio.com/images2/PC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87326-img0007.jpg)
![Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human duodenum shows high expression.](http://images.novusbio.com/images2/PC6-Antibody-Immunohistochemistry-Paraffin-NBP1-87326-img0008.jpg)
![Immunohistochemistry-Paraffin: PC6 Antibody [NBP1-87326] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/images2/PC6-Antibody-Immunohistochemistry-Paraffin-NBP1-87326-img0009.jpg)
![Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in Hela S3 NE (Cat # L013V3 ).](http://images.novusbio.com/images2/PACE4-Antibody-Western-Blot-H00005046-A01-img0002.jpg)
![Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in NIH/3T3.](http://images.novusbio.com/images2/PACE4-Antibody-Western-Blot-H00005046-A01-img0003.jpg)
![Western Blot: PACE4 Antibody [H00005046-A01] - Analysis of PCSK6 expression in Raw 264.7.](http://images.novusbio.com/images2/PACE4-Antibody-Western-Blot-H00005046-A01-img0005.jpg)
![Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.](http://images.novusbio.com/images2/SUB1-Antibody-Immunohistochemistry-Paraffin-NBP1-82454-img0012.jpg)
![Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/images2/SUB1-Antibody-Immunohistochemistry-Paraffin-NBP1-82454-img0013.jpg)
![Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human tonsil shows strong nuclear positivity in non - germinal center cells.](http://images.novusbio.com/images2/SUB1-Antibody-Immunohistochemistry-Paraffin-NBP1-82454-img0014.jpg)
![Immunohistochemistry-Paraffin: Secretogranin V Antibody [NBP1-90312] - Analysis in human adrenal gland and skeletal muscle tissues using NBP1-90312 antibody. Corresponding Secretogranin V RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Secretogranin-V-Antibody-Immunohistochemistry-Paraffin-NBP1-90312-img0010.jpg)
![Immunohistochemistry-Paraffin: Secretogranin V Antibody [NBP1-90312] - Staining of human lymphoid tissues, pancreas, pituitary gland and skeletal muscle using Anti-Secretogranin V antibody NBP1-90312 (A) shows similar protein distribution across tissues to independent antibody NBP2-68983 (B).](http://images.novusbio.com/images2/Secretogranin-V-Antibody-Immunohistochemistry-Paraffin-NBP1-90312-img0009.jpg)
![Immunohistochemistry-Paraffin: Secretogranin V Antibody [NBP1-90312] - Staining of human pituitary gland shows strong cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/images2/Secretogranin-V-Antibody-Immunohistochemistry-Paraffin-NBP1-90312-img0012.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")