Proprotein Convertase 2/PCSK2 Antibody (694009)

Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Human Proprotein Convertase 2/PCSK2 Monoclonal Antibody (Catalog # MAB6018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proprotein Convertase 2/PCSK2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Proprotein Convertase 2/ PCSK2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Proprotein Convertase 2/ PCSK2 Monoclonal Antibody (Catalog # MAB6018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IP, ICC/IF
• Clone: 694009
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Proprotein Convertase 2/PCSK2 Antibody (694009) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Proprotein Convertase 2/PCSK2
  Gly110-Asn638
  Accession # P16519
• Specificity: Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Proprotein Convertase 1, 7, 9, or rhFurin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCSK2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proprotein Convertase 2/PCSK2 Antibody (694009)

• EC 3.4.21
• EC 3.4.21.94
• KEX2-like endoprotease 2
• NEC 2
• NEC2
• NEC2SPC2
• neuroendocrine convertase 2
• PC2
• PC2NEC-2
• PCSK2
• Prohormone convertase 2
• Proprotein Convertase 2
• proprotein convertase subtilisin/kexin type 2
• SPC2

Background

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

1. Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
2. Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
3. Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
4. Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
5. Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proprotein Convertase 2/PCSK2 Antibody (MAB6018)(2)

Publications using MAB6018

• Chinmai Patibandla, Zahidul Islam Khan, Louise MacGregor, Mark James Campbell, Steven Patterson Costus pictus D. Don leaf extract stimulates GLP-1 secretion from GLUTag L-cells and has cytoprotective effects in BRIN-BD11 beta -cells Journal of Ethnopharmacology 2020-10-05 [PMID: 32422353]
• Masaaki Hokama, Sugako Oka, Julio Leon, Toshiharu Ninomiya, Hiroyuki Honda, Kensuke Sasaki et al. Altered Expression of Diabetes-Related Genes in Alzheimer's Disease Brains: The Hisayama Study Cerebral Cortex 2014-09-01 [PMID: 23595620]

Bioinformatics

• Gene Symbol: PCSK2
• Uniprot:
  • P16519(Human)