Proprotein Convertase 2/PCSK2 Antibody (694009) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IP, ICC/IF
• Clone: 694009
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Proprotein Convertase 2/PCSK2 Antibody (694009) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Proprotein Convertase 2/PCSK2
  Gly110-Asn638
  Accession # P16519
• Specificity: Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Proprotein Convertase 1, 7, 9, or rhFurin is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proprotein Convertase 2/PCSK2 Antibody (694009) [Alexa Fluor® 488]

• EC 3.4.21
• EC 3.4.21.94
• KEX2-like endoprotease 2
• NEC 2
• NEC2
• NEC2SPC2
• neuroendocrine convertase 2
• PC2
• PC2NEC-2
• PCSK2
• Prohormone convertase 2
• Proprotein Convertase 2
• proprotein convertase subtilisin/kexin type 2
• SPC2

Background

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.