PRMT5 Antibody

Western Blot: PRMT5 Antibody [NB110-40571] - Detection of Human and Mouse PRMT5 on HeLa whole cell lysate using NB110-40571. PRMT5 was also immunoprecipitated by another rabbit anti-PRMT5 antibodies and NB110-40570.

Immunohistochemistry-Paraffin: PRMT5 Antibody [NB110-40571] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PRMT5 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Bv, Pm, Xp
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PRMT5 Antibody Summary

• Description: Novus Biologicals Rabbit PRMT5 Antibody (NB110-40571) is a polyclonal antibody validated for use in IHC and WB. Anti-PRMT5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 580 and the C-terminus (residue 637) of Protein Arginine Methyltransferase 5 using the numbering given in entry NP_006100.2 (GeneID 10419).
• Predicted Species: Primate (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000-1:10000
• Application Notes: Not recommended for Immunoprecipitation, use NB110-40570. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PRMT5 Overexpression Lysate

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRMT5 Antibody

• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.320
• Histone-arginine N-methyltransferase PRMT5
• HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein
• HRMT1L5
• IBP72
• Jak-binding protein 1
• JBP1
• PRMT5
• protein arginine methyltransferase 5
• protein arginine N-methyltransferase 5
• Shk1 kinase-binding protein 1 homolog
• skb1 (S. pombe) homolog
• SKB1 homolog (S. pombe)
• SKB1 homolog
• SKB1
• SKB1HsSKB1 homolog

Background

Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT5 Antibody (NB110-40571)(1)

Publication using NB110-40571

• Pak MG, Lee HW, Roh MS. High nuclear expression of protein arginine methyltransferase-5 is a potentially useful marker to estimate submucosal invasion in endoscopically resected early colorectal carcinoma. Pathol. Int. 2015-08-06 [PMID: 26248553] (IHC-P, Human)
  
  Details:
  PRMT5 antibody was used for IHC-P analysis of endoscopically resected tissue samples from human suvjects having adenomas with low-grade dysplasia, adenomas with high-grade dysplasia, intramucosal carcinomas, and submucosal invasive carcinomas. The primary antibody was used at 1:500 dilution and the assay was perfomred on automated stainer (Figure 1).

Bioinformatics

• Gene Symbol: PRMT5
• Entrez:
  • 10419(Human)
• Uniprot:
  • O14744(Human)