PRMT3 Antibody (PRMT3-367) - BSA Free

Western Blot: PRMT3 Antibody (PRMT3-367) - BSA Free [NB120-12190] - Whole cells extract of 293T cell line transfected with human PRMT3 were separated on SDS-PAGE and probed with Monoclonal Anti-PRMT3 antibody produced in Mouse, Clone: PRMT3-367. The antibody was developed using 1:5000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.

Product Discontinued

PRMT3 Antibody (PRMT3-367) - BSA Free Summary

• Immunogen: Synthetic peptide: TVHKNKKDPRSLIV conjugated to KLH, corresponding to amino acids 501-514 of Rat PRMT3.
• Epitope: The antibody epitope resides within amino acids 501-514 of rat PRMT3.
• Localization: Cytoplasmic
• Specificity: does not cross react with PRMT1, 2, 4, 5, or 6.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRMT3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. Western Blot: Use at a concentration of 1-2 ug/ml. This concentration is recommended using a whole cell extract of the 293T cell line transfected with human PRMT3. Predicted molecular weight: 58 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for PRMT3 Antibody (PRMT3-367) - BSA Free

• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.319
• heterogeneous nuclear ribonucleoprotein methyltransferase-like 3
• Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
• HMT1 hnRNP methyltransferase-like 3 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 3
• HRMT1L3
• PRMT3
• protein arginine methyltransferase 3
• protein arginine N-methyltransferase 3

Background

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRMT3
• Entrez:
  • 10196(Human)
  • 71974(Mouse)
  • 89820(Rat)