PRKD3/nPKC nu Antibody - BSA Free

Western Blot: Protein Kinase D3 Antibody [NBP2-19958] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Protein Kinase D3 Antibody [NBP2-19958] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PRKD3/nPKC nu Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRKD3/nPKC nu. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (96%), Rhesus Macaque (99%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKD3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PRKD3/nPKC nu Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.13
• EPK2
• EPK2PKD3
• nPKC nu
• nPKC-nu
• PKC nu
• PKC-NU
• PRKCN
• PRKCNnPKC-NU
• PRKD3
• Protein kinase C nu type
• protein kinase C, nu
• protein kinase D3
• Protein kinase EPK2
• protein-serine/threonine kinase
• serine/threonine-protein kinase D3

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PRKD3
• Uniprot:
  • O94806(Human)