| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38492-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38511-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16319-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22452-50ul | |||
![Western Blot: PRKCSH Antibody [NBP2-19931] - Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Western-Blot-NBP2-19931-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PRKCSH Antibody [NBP2-19931] - Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP2-19931] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19931-img0004.jpg)
![Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-19931] - Paraffin-embedded U87 xenograft. PRKCSH antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-19931-img0002.jpg)
![Western Blot: PRKCSH Antibody [NBP2-19931] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Western-Blot-NBP2-19931-img0001.jpg)
![Western Blot: PRKCSH Antibody [NBP1-32006] - Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Western-Blot-NBP1-32006-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PRKCSH Antibody [NBP1-32006] - Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP1-32006] - PRKCSH antibody detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32006-img0006.jpg)
![Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP1-32006] - Normal liver.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP1-32006-img0004.jpg)
![Western Blot: PRKCSH Antibody [NBP1-32006] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Western-Blot-NBP1-32006-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP1-32006] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32006-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP2-38492] - Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38492-img0003.jpg "Immunocytochemistry/Immunofluorescence: PRKCSH Antibody [NBP2-38492] - Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38492] - Staining in human thyroid gland and skeletal muscle tissues using anti-PRKCSH antibody. Corresponding PRKCSH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-38492-img0006.jpg)
![Immunohistochemistry: PRKCSH Antibody [NBP2-38492] - Staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-NBP2-38492-img0002.jpg)
![Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38492] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-38492-img0004.jpg)
![Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38492] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-38492-img0005.jpg)
![Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38511] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-38511-img0003.jpg "Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38511] - Staining of human thyroid gland shows high expression.")
![Immunohistochemistry: PRKCSH Antibody [NBP2-38511] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-NBP2-38511-img0001.jpg)
![Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38511] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-38511-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PRKCSH Antibody [NBP2-38511] - Staining in human thyroid gland and skeletal muscle tissues using anti-PRKCSH antibody. Corresponding PRKCSH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Immunohistochemistry-Paraffin-NBP2-38511-img0004.jpg)
![Western Blot: PRKCSH Antibody [NBP3-16319] - Western blot analysis of extracts of various cell lines, using PRKCSH antibody (NBP3-16319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Western-Blot-NBP3-16319-img0001.jpg "Western Blot: PRKCSH Antibody [NBP3-16319] - Western blot analysis of extracts of various cell lines, using PRKCSH antibody (NBP3-16319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: PRKCSH Antibody [NBP3-16319] - Western blot analysis of extracts of Mouse lung, using PRKCSH antibody (NBP3-16319) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-Western-Blot-NBP3-16319-img0002.jpg)
![Western Blot: PRKCSH Antibody (SR2231) [NBP3-22452] - Western blot analysis using NBP3-22452 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22452_rabbit-prkcsh-mab-sr2231-5720231456541.jpg "Western Blot: PRKCSH Antibody (SR2231) [NBP3-22452] - Western blot analysis using NBP3-22452 in A431 cell lysate.")
![Immunoprecipitation:PRKCSH AntibodyNBP3-30607] -Detection of human PRKCSH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody NBP3-30607 (lot NBP3-30607-1) used for IP at 6 µg per reaction. PRKCSH was also immunoprecipitated by rabbit anti-PRKCSH antibody BL18483. For blotting immunoprecipitated PRKCSH, NBP3-30607 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30607_rabbit-prkcsh-pab-301220241941542.jpg "Immunoprecipitation:PRKCSH AntibodyNBP3-30607] -Detection of human PRKCSH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRKCSH antibody NBP3-30607 (lot NBP3-30607-1) used for IP at 6 µg per reaction. PRKCSH was also immunoprecipitated by rabbit anti-PRKCSH antibody BL18483. For blotting immunoprecipitated PRKCSH, NBP3-30607 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: PRKCSH Overexpression Lysate (Adult Normal) [NBP2-08074] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRKCSH.](http://images.novusbio.com/fullsize/PRKCSH-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08074-img0001.jpg "Western Blot: PRKCSH Overexpression Lysate (Adult Normal) [NBP2-08074] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRKCSH.")
![Western Blot: PRKCSH Antibody (4Z3K3) [NBP3-15318] - Western blot analysis of extracts of various cell lines, using PRKCSH antibody (NBP3-15318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-4Z3K3-Western-Blot-NBP3-15318-img0001.jpg "Western Blot: PRKCSH Antibody (4Z3K3) [NBP3-15318] - Western blot analysis of extracts of various cell lines, using PRKCSH antibody (NBP3-15318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Sandwich ELISA: PRKCSH Antibody (3H7) [H00005589-M01] - Detection limit for recombinant GST tagged PRKCSH is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PRKCSH-Antibody-3H7-Sandwich-ELISA-H00005589-M01-img0001.jpg "Sandwich ELISA: PRKCSH Antibody (3H7) [H00005589-M01] - Detection limit for recombinant GST tagged PRKCSH is 3 ng/ml as a capture antibody.")
![Western Blot: PRKCSH Antibody (A6H9-R) [NBP3-32386] - Western blot analysis of PRKCSH on different lysates with Mouse anti-PRKCSH antibody (NBP3-32386) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Daudi cell lysate Lane 4: Jurkat cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 59 kDa Observed band size: 80 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32386) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32386_mouse-prkcsh-mab-a6h9-r-278202415242662.jpg "Western Blot: PRKCSH Antibody (A6H9-R) [NBP3-32386] - Western blot analysis of PRKCSH on different lysates with Mouse anti-PRKCSH antibody (NBP3-32386) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Daudi cell lysate Lane 4: Jurkat cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 59 kDa Observed band size: 80 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32386) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")