PRKAG3 Antibody

Product Discontinued

PRKAG3 Antibody Summary

• Immunogen: Peptide with sequence C-KLGWDDELRKPGAQ corresponding to internal region according to NP_059127.2.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRKAG3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments in human heart, skeletal muscle and pancreas lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for PRKAG3 Antibody

• 5'-AMP-activated protein kinase subunit gamma-3
• AMPK gamma 3
• AMPK gamma-3 chain
• AMPK gamma3
• AMPK subunit gamma-3
• AMPKG3
• AMPKG3,5'-AMP-activated protein kinase, gamma-3 subunit
• PRKAG3
• protein kinase, AMP-activated, gamma 3 non-catalytic subunit

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRKAG3 Antibody (NBP1-28491)(1)

Publication using NBP1-28491

• Costford SR, Kavaslar N, Ahituv N et al. Gain-of-function R225W mutation in human AMPKgamma3 causing increased glycogen and decreased triglyceride in skeletal muscle. PLoS ONE 2007-09-19 [PMID: 17878938]

Bioinformatics

• Gene Symbol: PRKAG3
• Entrez:
  • 53632(Human)
  • 319974(Mouse)
• Uniprot:
  • NP_059127.2(Human)