PRKAG2 Antibody

Western Blot: PRKAG2 Antibody [H00051422-B01P] - Analysis of PRKAG2 expression in transfected 293T cell line by PRKAG2 polyclonal antibody. Lane 1: PRKAG2 transfected lysate(36.08 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody [H00051422-B01P] - Analysis of purified antibody to PRKAG2 on HeLa cell. (antibody concentration 10 ug/ml)

Product Discontinued

PRKAG2 Antibody Summary

• Immunogen: PRKAG2 (NP_077747.1, 1 a.a. - 328 a.a.) full-length human protein. MLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
• Specificity: PRKAG2 - protein kinase, AMP-activated, gamma 2 non-catalytic subunit,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PRKAG2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRKAG2 Antibody

• AAKG
• AAKG2
• AMPK gamma2
• CMH6AMPK subunit gamma-2
• H91620p
• H91620pAMPK gamma2
• PRKAG2
• protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• WPWS5'-AMP-activated protein kinase subunit gamma-2

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAG2