PRAK Antibody (OTI5E1)

Western Blot: PRAK Antibody (5E1) [NBP2-45753] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRAK.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI5E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PRAK Antibody (OTI5E1) Summary

• Description: Novus Biologicals Mouse PRAK Antibody (OTI5E1) (NBP2-45753) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 197-471 of human MAPKAPK5 (NP_003659) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPKAPK5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRAK Antibody (OTI5E1)

• EC 2.7.11
• MAP kinase-activated protein kinase 5
• MAPK-activated protein kinase 5
• MAPKAP kinase 5
• MAPKAPK-5
• mitogen-activated protein kinase-activated protein kinase 5
• p38-regulated/activated protein kinase
• PRAKEC 2.7.11.1

Background

PRAK is encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK5