PPP2R5A Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

PPP2R5A Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PPP2R5A.

  Source: E. coli

  Amino Acid Sequence: TLNELVEYVSTNRGVIVESAYSD

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: PPP2R5A
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-57154.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for PPP2R5A Recombinant Protein Antigen

• B56A
• MGC131915
• PP2A B subunit isoform B56-alpha
• PP2A B subunit isoform B'-alpha
• PP2A B subunit isoform PR61-alpha
• PP2A B subunit isoform R5-alpha
• PP2A, B subunit, B' alpha isoform
• PP2A, B subunit, B56 alpha isoform
• PP2A, B subunit, PR61 alpha isoform
• PP2A, B subunit, R5 alpha isoform
• PR61A
• PR61alpha
• protein phosphatase 2, regulatory subunit B (B56), alpha isoform
• protein phosphatase 2, regulatory subunit B', alpha isoform
• protein phosphatase 2, regulatory subunit B', alpha
• serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alphaisoform
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Background

PPP2R5A belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity and might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PPP2R5A