PPP2R1A Antibody

Western Blot: PPP2R1A Antibody [NB100-41408] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPPP2R1A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: PPP2R1A Antibody [NB100-41408] - Detection of human PPP2R1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP2R1A antibody NB100-41408 used for IP at 3 ug per reaction. PPP2R1A was also immunoprecipitated by rabbit anti-PPP2R1A antibody NB100-41407. For blotting immunoprecipitated PPP2R1A, NB100-41408 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PPP2R1A Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 540 and the C-terminus (residue 588) of human protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform using the numbering given in entry NP_055040.2
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP2R1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PPP2R1A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R1A Antibody

• Medium tumor antigen-associated 61 kDa protein
• MGC786
• PP2A subunit A isoform PR65-alpha
• PP2A subunit A isoform R1-alpha
• PP2AAALPHA
• PP2A-Aalpha
• PR65A
• protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform
• protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
• protein phosphatase 2, regulatory subunit A, alpha
• serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform
• serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform

Background

PPP2R1A encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R1A Antibody (NB100-41408)(1)

Publication using NB100-41408

• Quintela-Fandino M, Arpaia E, Brenner D et al. HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. Proc Natl Acad Sci U S A 2010-02-01 [PMID: 20133759]

Bioinformatics

• Gene Symbol: PPP2R1A
• Entrez:
  • 5518(Human)
  • 51792(Mouse)
• Uniprot:
  • P30153(Human)