PPP2R1A Overexpression Lysate (Native)

Images

 
Western Blot: PPP2R1A Overexpression Lysate (Adult Normal) [NBL1-14692] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPP2R1A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PPP2R1A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200056 and based on accession number NM_014225. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform (PPP2R1A), mRNA.
Gene
PPP2R1A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
65.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PPP2R1A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPP2R1A Overexpression Lysate (Native)

  • Medium tumor antigen-associated 61 kDa protein
  • MGC786
  • PP2A subunit A isoform PR65-alpha
  • PP2A subunit A isoform R1-alpha
  • PP2AAALPHA
  • PP2A-Aalpha
  • PR65A
  • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform
  • protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
  • protein phosphatase 2, regulatory subunit A, alpha
  • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform
  • serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform

Background

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PPP2R1A Lysate (NBL1-14692) (0)

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Bioinformatics

Gene Symbol PPP2R1A