![Western Blot: PPHLN1 Antibody [NBP2-81775] - Analysis of PPHLN1 in mouse colon tissue lysate with PPHLN1 Antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize2/PPHLN1-Antibody-Western-Blot-NBP2-81775-img0001.jpg "Western Blot: PPHLN1 Antibody [NBP2-81775] - Analysis of PPHLN1 in mouse colon tissue lysate with PPHLN1 Antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | PPHLN1 antibody was raised against a 16 amino acid synthetic peptide near the center of human PPHLN1. The immunogen is located within amino acids 160 - 210 of PPHLN1. Amino Acid Squence: SYKRQNEGNPERDKER |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPHLN1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-13795] - Analysis in human esophagus and skeletal muscle tissues using NBP2-13795 antibody. Corresponding Periplakin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Periplakin Antibody-Immunohistochemistry-Paraffin-NBP2-13795-img0007.jpg)
![Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-13795] - Staining of human esophagus shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/images2/Periplakin-Antibody-Immunohistochemistry-Paraffin-NBP2-13795-img0009.jpg)
![Immunohistochemistry-Paraffin: Periplakin Antibody [NBP2-13795] - Staining of human skin shows weak to moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/images2/Periplakin-Antibody-Immunohistochemistry-Paraffin-NBP2-13795-img0010.jpg)
![Immunohistochemistry-Paraffin: Synphilin-1 Antibody [NBP2-55955] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/images2/Synphilin-1 Antibody-Immunohistochemistry-Paraffin-NBP2-55955-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Synphilin-1 Antibody [NBP2-55955] - Staining of human cell line RT4 shows localization to cytoplasmic bodies.](http://images.novusbio.com/images2/Synphilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55955-img0001.jpg)
![Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse cerebellum. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559 diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/images2/alpha-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP2-15365-img0018.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/alpha-Synuclein-Antibody-Western-Blot-NBP2-15365-img0022.jpg)
![Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.](http://images.novusbio.com/images2/alpha-Synuclein-Antibody-Immunohistochemistry-Frozen-NBP2-15365-img0024.jpg)
![Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded human appendix using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/images2/Galectin-4-Antibody-(9Z2B5)-Immunohistochemistry-Paraffin-NBP3-16249-img0001.jpg)
![Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded mouse colon using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/images2/Galectin-4-Antibody-(9Z2B5)-Immunohistochemistry-Paraffin-NBP3-16249-img0002.jpg)
![Immunohistochemistry: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunofluorescence analysis of rat rectum using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Galectin-4-Antibody-(9Z2B5)-Immunohistochemistry-NBP3-16249-img0003.jpg)
![Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/HDAC1-Antibody-Knockout-Validated-NB100-56340-img0007.jpg)
![Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/HDAC1-Antibody-Simple-Western-NB100-56340-img0003.jpg)
![Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.](http://images.novusbio.com/images2/HDAC1-Antibody-Western-Blot-NB100-56340-img0002.jpg)
![Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Flow-Cytometry-NB100-56708-img0043.jpg)
![Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Flow-Cytometry-NB100-56708-img0045.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0044.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for PPHLN1 Antibody (NBP2-81775)Discover more about diseases related to PPHLN1 Antibody (NBP2-81775).
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| Gene Symbol | PPHLN1 |
![Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP2-81775] - Immunofluorescence of PPHLN1 in human colon tissue with PPHLN1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize2/PPHLN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81775-img0003.jpg "Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP2-81775] - Immunofluorescence of PPHLN1 in human colon tissue with PPHLN1 antibody at 5 ug/mL.")
![Immunohistochemistry: PPHLN1 Antibody [NBP2-81775] - Immunohistochemistry of PPHLN1 in human colon tissue with PPHLN1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize2/PPHLN1-Antibody-Immunohistochemistry-NBP2-81775-img0002.jpg "Immunohistochemistry: PPHLN1 Antibody [NBP2-81775] - Immunohistochemistry of PPHLN1 in human colon tissue with PPHLN1 antibody at 2.5 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Caspase 5 Antibody [NBP1-77209] - P815 cells with Caspase-5 antibody at 2 ug/mL.](http://images.novusbio.com/images2/Caspase-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77209-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase 5 Antibody [NBP1-77209] - P815 cells with caspase-5 antibody at 2 ug/ml.](http://images.novusbio.com/images2/Caspase-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77209-img0002.jpg)
![Western Blot: Caspase 5 Antibody [NBP1-77209] - Analysis of Caspase 5 in Ramos cells with anti-Caspase 5 (IN) using NBP1-77209 at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/images2/Caspase-5-Antibody-Western-Blot-NBP1-77209-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human stomach, upper shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/CDC7-Antibody-Immunohistochemistry-Paraffin-NBP2-32708-img0002.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.](http://images.novusbio.com/images2/CDC7-Antibody-Immunohistochemistry-Paraffin-NBP2-32708-img0008.jpg)
![Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/CDC7-Antibody-Immunohistochemistry-Paraffin-NBP2-32708-img0009.jpg)
![Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/HDAC9-Antibody-Western-Blot-NBP2-03993-img0004.jpg)
![Vitronectin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Vitronectin [Unconjugated]")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")