PPAP2B Antibody

Western Blot: PPAP2B Antibody [NBP1-02866] - Bacterially expressed LPP3 protein when untreated (-) and treated with with 0.1 mM IPTG (iso propyl-beta-D- thiogalactopyranoside) (+) at a dilution of 10 ug/ml.

Immunohistochemistry-Paraffin: PPAP2B Antibody [NBP1-02866] - Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Product Discontinued

PPAP2B Antibody Summary

• Immunogen: A synthetic peptide of PPAP2B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLPP3
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 20 ug/ml
  • Immunohistochemistry-Paraffin 20 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for PPAP2B Antibody

• Dri42
• EC 3.1.3.4
• lipid phosphate phosphohydrolase 3
• LPP3PAP2 beta
• MGC15306
• PAP2b
• PAP-2b
• PAP2-beta
• Phosphatidate phosphohydrolase type 2b
• Phosphatidic acid phosphatase 2b
• phosphatidic acid phosphatase type 2B
• type-2 phosphatidic acid phosphatase-beta
• vascular endothelial growth factor and type I collagen inducible
• Vascular endothelial growth factor and type I collagen-inducible protein
• VCIP

Background

The protein encoded by the PPAP2B gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. Alternatively spliced transcript variants encoding the same protein have been described. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPAP2B Antibody (NBP1-02866)(1)

Publication using NBP1-02866

• Mkannez G, Gagne-Ouellet V, Nsaibia MJ et al. DNA methylation of a PLPP3 MIR transposon-based enhancer promotes an osteogenic program in calcific aortic valve disease Cardiovasc. Res. 2018-05-02 [PMID: 29726894] (WB, Human)

Bioinformatics

• Gene Symbol: PLPP3
• Entrez:
  • 8613(Human)
• Uniprot:
  • O14495(Human)