PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

Images

 
Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody (M01), clone 1A3-2A4.Lane 1: PPM1B transfected lysate(52.643 ...read more
Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of monoclonal antibody to PPM1B on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi ( Cat # H00005495-R01V ) (Lane 2) or non-transfected ...read more
Proximity Ligation Assay: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of protein-protein interactions between ARRB2 and PPM1B. HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 ...read more
Immunoprecipitation: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPM1B monoclonal ...read more
ELISA: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Detection limit for recombinant GST tagged PPM1B is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IP, KD
Clone
1A3-2A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PPM1B (AAH12002, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI
Specificity
PPM1B - protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPM1B
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Proximity Ligation Assay
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein and transfected lysate for WB. It has also been used for IF, RNAi Validation and ELISA.
Publications
Read Publication using
H00005495-M01 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PP2C beta/PPM1B Antibody (1A3-2A4) - Azide and BSA Free

  • EC 3.1.3.16
  • MGC21657
  • PP2C beta
  • Pp2c2
  • PP2CB
  • PP2C-beta
  • PP2C-beta-X
  • PP2CBmagnesium-dependent, beta isoform
  • PPM1B
  • protein phosphatase 1B
  • Protein phosphatase 2C isoform beta
  • protein phosphatase 2C-like protein
  • protein phosphatase, Mg2+/Mn2+ dependent, 1B

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PP2C beta/PPM1B Antibody (H00005495-M01)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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(1)
WB
(1)
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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPM1B
Entrez
OMIM
Uniprot