Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B expression in transfected 293T cell line by PPM1B monoclonal antibody (M01), clone 1A3-2A4.Lane 1: PPM1B transfected lysate(52.643 ...read more
Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of monoclonal antibody to PPM1B on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B over-expressed 293 cell line, cotransfected with PPM1B Validated Chimera RNAi ( Cat # H00005495-R01V ) (Lane 2) or non-transfected ...read more
Proximity Ligation Assay: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of protein-protein interactions between ARRB2 and PPM1B. HeLa cells were stained with anti-ARRB2 rabbit purified polyclonal 1:1200 ...read more
Immunoprecipitation: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Analysis of PPM1B transfected lysate using anti-PPM1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPM1B monoclonal ...read more
ELISA: PP2C beta/PPM1B Antibody (1A3-2A4) [H00005495-M01] - Detection limit for recombinant GST tagged PPM1B is 0.3 ng/ml as a capture antibody.
PPM1B (AAH12002, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI
Specificity
PPM1B - protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPM1B
Purity
IgG purified
Innovator's Reward
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Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified
Notes
Quality control test: Antibody Reactive Against Recombinant Protein.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for PP2C beta/PPM1B Antibody (1A3-2A4)
EC 3.1.3.16
MGC21657
PP2C beta
Pp2c2
PP2CB
PP2C-beta
PP2C-beta-X
PP2CBmagnesium-dependent, beta isoform
PPM1B
protein phosphatase 1B
Protein phosphatase 2C isoform beta
protein phosphatase 2C-like protein
protein phosphatase, Mg2+/Mn2+ dependent, 1B
Background
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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