PP2C alpha/PPM1A Antibody (p6c7) - BSA Free

Western Blot: PP2C alpha/PPM1A Antibody (p6c7) [NBP1-04333] - Effect of NOX1 knockdown on phosphatase activity and expression in HT-29 cells. Western analysis demonstrates the protein expression and phosphorylation status of several members of the protein-tyrosine and serine/threonine phosphatase families, respectively, in HT-29, SC, and 6A cells. As described under -Experimental procedures,

Western Blot: PP2C alpha/PPM1A Antibody (p6c7) [NBP1-04333] - Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2Calpha(1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PP2Calpha(~43kDa).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: p6c7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PP2C alpha/PPM1A Antibody (p6c7) - BSA Free Summary

• Immunogen: Recombinant human PP2C alpha/PPM1A (1-382aa) purified from E. coli (NP_066283).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:250-1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PP2C alpha/PPM1A Antibody (p6c7) - BSA Free

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C(PP2Calpha) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNK MAPK pathways and regulates cellular stress response in eukaryotes. The PPM(metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2C, the prototypical member of the PPM family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2C alpha/PPM1A Antibody (NBP1-04333)(1)

Publication using NBP1-04333

• Juhasz A, Markel S, Gaur S et al. NADPH Oxidase 1 Supports Proliferation of Colon Cancer Cells by Modulating Reactive Oxygen Species-Dependent Signal Transduction. J. Biol. Chem. 2017-03-22 [PMID: 28330872] (WB, Human)

Bioinformatics

• Gene Symbol: PPM1A
• Entrez:
  • 5494(Human)
  • 19042(Mouse)
• Uniprot:
  • P35813()