PP2C alpha/PPM1A Antibody (p6c7) - BSA Free

Images

 
Western Blot: PP2C alpha/PPM1A Antibody (p6c7) [NBP1-04333] - Effect of NOX1 knockdown on phosphatase activity and expression in HT-29 cells. Western analysis demonstrates the protein expression and phosphorylation ...read more
Western Blot: PP2C alpha/PPM1A Antibody (p6c7) [NBP1-04333] - Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2Calpha(1:250). Proteins were visualized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
p6c7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

PP2C alpha/PPM1A Antibody (p6c7) - BSA Free Summary

Immunogen
Recombinant human PP2C alpha/PPM1A (1-382aa) purified from E. coli (NP_066283).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPM1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:250-1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-04333 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PP2C alpha/PPM1A Antibody (p6c7) - BSA Free

  • EC 3.1.3.16
  • FLJ42306
  • MGC9201
  • MMPa-2
  • MPPa-1
  • phosphatase 2C alpha
  • PP2C alpha
  • Pp2c1
  • PP2CA
  • PP2Calpha
  • PP2C-ALPHA
  • PPM1A
  • PPPM1A
  • protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
  • protein phosphatase 1A
  • protein phosphatase 2C alpha isoform
  • Protein phosphatase 2C isoform alpha
  • Protein phosphatase IA
  • protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C(PP2Calpha) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNK MAPK pathways and regulates cellular stress response in eukaryotes. The PPM(metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2C, the prototypical member of the PPM family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PP2C alpha/PPM1A Antibody (NBP1-04333)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for PP2C alpha/PPM1A Antibody (NBP1-04333)

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Bioinformatics

Gene Symbol PPM1A
Entrez
Uniprot