PP2C alpha/PPM1A Antibody (498922) [Alexa Fluor™ Plus 405]

• Clone: 498922
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

PP2C alpha/PPM1A Antibody (498922) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human, mouse, and rat PP2C alpha /PPM1A in Western blots. No cross-reactivity with recombinant human PP2C beta , ILKAP/PP2Cδ, or PP2C gamma is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2C alpha/PPM1A Antibody (498922) [Alexa Fluor™ Plus 405]

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C alpha, also called PP2C alpha , Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues (1). It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid, (2) but has an absolute requirement for either magnesium or manganese ions (3) and can be activated by unsaturated fatty acids such as arachidonic and oleic acids (4). Overexpression of PP2C alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 (5). Dephosphorylation of proteins in the nucleus by PP2C alpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS (6) and stress-activated proteins such as p38 and JNK (7).

1. Donella-Deanna, A. et al. (1991) Biochimica et Biophysica Acta 1094:130.
2. Bialojan, C. and A. Takai (1998) Biochem. J. 256:283.
3. Cohen, P. Annu. Rev. Biochem. (1989) 58:453.
4. Klumpp, S. et al. (1998) FEBS Let. 437:229.
5. Ofek, P. et al. (2003) J. Biol. Chem. 278:14299.
6. Lin, X. et al. (2006) Cell 125:915.
7. Takekawa, M. et al. (1998) EMBO J. 17:4744.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.