PP2C alpha/PPM1A Antibody (498922)

Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PP2Ca/ PPM1A Monoclonal Antibody (Catalog # MAB4150) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2Ca/PPM1A at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 498922
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PP2C alpha/PPM1A Antibody (498922) Summary

• Immunogen: E. coli-derived recombinant human PP2C alpha /PPM1A
  Met1-Gly324
  Accession # P35813
• Specificity: Detects human, mouse, and rat PP2C alpha /PPM1A in Western blots. No cross-reactivity with recombinant human PP2C beta , ILKAP/PP2Cδ, or PP2C gamma is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2C alpha/PPM1A Antibody (498922)

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C alpha, also called PP2C alpha , Protein Phosphatase, Magnesium-dependent type 1A, and PPM1A, dephosphorylates proteins with some preference for phospho-threonine over phospho-serine residues (1). It is insensitive to the PP1 and PP2a inhibitors such as okadaic acid, (2) but has an absolute requirement for either magnesium or manganese ions (3) and can be activated by unsaturated fatty acids such as arachidonic and oleic acids (4). Overexpression of PP2C alpha causes G2/M cell cycle arrest and apoptosis that has been associated with the activation of p53 (5). Dephosphorylation of proteins in the nucleus by PP2C alpha is suspected to play a role in terminating or reducing the sensitivity of the responses to SMADS (6) and stress-activated proteins such as p38 and JNK (7).

1. Donella-Deanna, A. et al. (1991) Biochimica et Biophysica Acta 1094:130.
2. Bialojan, C. and A. Takai (1998) Biochem. J. 256:283.
3. Cohen, P. Annu. Rev. Biochem. (1989) 58:453.
4. Klumpp, S. et al. (1998) FEBS Let. 437:229.
5. Ofek, P. et al. (2003) J. Biol. Chem. 278:14299.
6. Lin, X. et al. (2006) Cell 125:915.
7. Takekawa, M. et al. (1998) EMBO J. 17:4744.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2C alpha/PPM1A Antibody (MAB4150)(1)

Publication using MAB4150

• D Medina-Ver, JA Navarro, R Tovar, C Rosell-Val, A Gutiérrez-, JC Ledesma, C Sanjuan, FJ Pavón, E Baixeras, F Rodríguez, J Decara Activation of PI3K/Akt Signaling Pathway in Rat Hypothalamus Induced by an Acute Oral Administration of D-Pinitol Nutrients, 2021-06-30;13(7):. 2021-06-30 [PMID: 34209137] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: PPM1A
• Uniprot:
  • P35813()