PP2A alpha Antibody (Y119)

Product Discontinued

PP2A alpha Antibody (Y119) Summary

• Immunogen: A synthetic peptide corresponding to residues near C-terminus of human PP2A was used as immunogen.
• Specificity: This antibody detects both alpha and beta isoforms of PP2A.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPP2CA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:100
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 36 kDa.
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PP2A alpha Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for PP2A alpha Antibody (Y119)

• EC 3.1.3.16
• PP2A
• PP2A-alpha
• PP2Ac
• PP2CA
• PP2Calpha
• PPP2CA
• protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• protein phosphatase 2, catalytic subunit, alpha isozyme
• protein phosphatase 2A catalytic subunit, alpha isoform
• Replication protein C
• RP-C
• serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
• serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2A alpha Antibody (NB110-57430)(1)

Publication using NB110-57430

• Singh AP, Bafna S, Chaudhary K et al. Genome-wide expression profiling reveals transcriptomic variation and perturbed gene networks in androgen-dependent and androgen-independent prostate cancer cells . Cancer Letters 259 28-38. 2008-01-01 [PMID: 17977648] (WB, IF/IHC)

Bioinformatics

• Gene Symbol: PPP2CA
• Entrez:
  • 5515(Human)
  • 19052(Mouse)
  • 24672(Rat)
• Uniprot:
  • P67775()