Recombinant Human PP1 Inhibitor-2 His Protein

SDS-Page: Recombinant Human PP1 Inhibitor-2 Protein [NBP2-23380]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human PP1 Inhibitor-2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-166 of Human PP1 Inhibitor-2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAASTAS HRPIKGILKN KTSTTSSMVA SAEQPRGNVD EELSKKSQKW DEMNILATYH PADKDYGLMK IDEPSTPYHS MMGDDEDACS DTEATEAMAP DILARKLAAA EGLEPKYRIQ EQESSGEEDS DLSPEEREKK RQFEMKRKLH YNEGLNIKLA RQLISKDLH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPP1R2
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human PP1 Inhibitor-2 His Protein

• IPP2
• IPP-2
• IPP2phosphatase inhibitor 2
• MGC87148
• phosphoprotein phosphatase
• PP1 Inhibitor2
• PP1 Inhibitor-2
• PPP1R2
• protein phosphatase 1, regulatory (inhibitor) subunit 2
• protein phosphatase inhibitor 2

Background

PPP1R2 is an inhibitor of protein-phosphatase 1 and forms heterodimer with PP1. Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex. Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex. Recombinant human PPP1R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R2