PP1 Inhibitor-2 Antibody

Western Blot: PP1 Inhibitor-2 Antibody [H00005504-D01P] - Analysis of PPP1R2 expression in transfected 293T cell line by PPP1R2 polyclonal antibody.Lane 1: PPP1R2 transfected lysate(23.00 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PP1 Inhibitor-2 Antibody [H00005504-D01P] - Analysis of antibody to PPP1R2 on HeLa cell. Antibody concentration 20 ug/ml.

Product Discontinued

PP1 Inhibitor-2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PPP1R2 (NP_006232.1, 1 a.a. - 205 a.a.) full-length human protein. MAASTASHRPIKGILKNKTSTTSSMVASAEQPRGNVDEELSKKSQKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMMGDDEDACSDTEATEAMAPDILARKLAAAEGLEPKYRIQEQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQLISKDLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNKLRSS
• Specificity: Reacts with protein phosphatase 1, regulatory (inhibitor) subunit 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PP1 Inhibitor-2 Antibody

• IPP2
• IPP-2
• IPP2phosphatase inhibitor 2
• MGC87148
• phosphoprotein phosphatase
• PP1 Inhibitor2
• PP1 Inhibitor-2
• PPP1R2
• protein phosphatase 1, regulatory (inhibitor) subunit 2
• protein phosphatase inhibitor 2

Background

Two inhibitors of protein phosphatase 1 (PP1) include the phosphatase inhibitor 1 (IPP-1) and phosphatase inhibitor 2 (IPP-2). IPP-2, also known as I-2, interacts with the catalytic subunit of PP1 to form the heterodimer PP1I. The PP1I complex is present in the cytosol of cells in a broad range of vertebrate and invertebrate species. Although the heterodimer itself is inactive, a reversible phosphorylation of IPP-2 at Thr 72 by glycogen-synthase-kinase (GSK3) initiates activation of the heterodimer complex in vitro. Phosphorylation of IPP-2 by casein kinase-II at Ser 86, Ser 120, and Ser 121 enhances the rate of phosphorylation by GSK3 at Thr 72 and effectively activates the heterodimer complex. Besides moderating PP1 activity, IPP-2 may play a role as a chaperone for the correct folding of PP1. The gene for human IPP-2 maps to chromosome 6 in the major histocompatiblity complex region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R2
• Entrez:
  • 5504(Human)
• Uniprot:
  • NP_006232.1(Human)