Recombinant Human PP1 alpha/PPP1A Protein

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SDS-Page: Recombinant Human PP1 alpha/PPP1A Protein [NBP1-72418]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.2 mg/ml

Order Details

Recombinant Human PP1 alpha/PPP1A Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-330 of Human PP1 alpha/PPP1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

Details of Functionality
Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PPP1CA
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
39.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-HCl (pH8.5),0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50% glycerol
Preservative
No Preservative
Concentration
0.2 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human PP1 alpha/PPP1A Protein

  • EC 3.1.3.16
  • PP1 alpha
  • PP1alpha
  • PP-1AMGC15877
  • PPP1A
  • PPP1AMGC1674
  • PPP1CA
  • protein phosphatase 1, catalytic subunit, alpha isoform
  • protein phosphatase 1, catalytic subunit, alpha isozyme
  • serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
  • serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol PPP1CA
Entrez