Recombinant Human PP1 alpha/PPP1A His Protein

SDS-Page: Recombinant Human PP1 alpha/PPP1A Protein [NBP1-72418] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.2 mg/ml

Recombinant Human PP1 alpha/PPP1A His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-330 of Human PP1 alpha/PPP1A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

• Details of Functionality: Specific activity >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPP1CA
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 39.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50% glycerol
• Preservative: No Preservative
• Concentration: 0.2 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human PP1 alpha/PPP1A His Protein

• EC 3.1.3.16
• PP1 alpha
• PP1alpha
• PP-1AMGC15877
• PPP1A
• PPP1AMGC1674
• PPP1CA
• protein phosphatase 1, catalytic subunit, alpha isoform
• protein phosphatase 1, catalytic subunit, alpha isozyme
• serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
• serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for PP1 alpha/PPP1A Recombinant Protein (NBP1-72418)(3)

Publications using NBP1-72418

• Kastian RF, Baba K, Kaewkascholkul N et al. Dephosphorylation of neural wiring protein shootin1 by PP1 phosphatase regulates netrin-1-induced axon guidance The Journal of biological chemistry 2023-04-10 [PMID: 37044214] (In vitro)
• Serpico AF, Febbraro F, Pisauro C, Grieco D Compartmentalized control of Cdk1 drives mitotic spindle assembly Cell reports 2022-01-25 [PMID: 35081344]
• Tohyama S, Kawada M, Muramatsu H Et Al. The potent protein phosphatase 2A inhibitors aminocytostatins: new derivatives of cytostatin The Journal of antibiotics 2021-07-21 [PMID: 34290381]

Bioinformatics

• Gene Symbol: PPP1CA