PP1 alpha/PPP1A Overexpression Lysate (Native)

Images

 
Western Blot: PPP1A Overexpression Lysate (Adult Normal) [NBP2-10998] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPP1A.

Product Details

Summary
Applications WB

Order Details

PP1 alpha/PPP1A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001008709. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens PPP1A, mRNA.
Gene
PPP1CA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PP1 alpha/PPP1A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PP1 alpha/PPP1A Overexpression Lysate (Native)

  • EC 3.1.3.16
  • PP1 alpha
  • PP1alpha
  • PP-1AMGC15877
  • PPP1A
  • PPP1AMGC1674
  • PPP1CA
  • protein phosphatase 1, catalytic subunit, alpha isoform
  • protein phosphatase 1, catalytic subunit, alpha isozyme
  • serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
  • serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PP1 alpha/PPP1A Lysate (NBP2-10998) (0)

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Bioinformatics

Gene Symbol PPP1CA
Entrez