POU6F1 Antibody (6H1) - Azide and BSA Free

Western Blot: POU6F1 Antibody (6H1) [H00005463-M01] - Analysis of POU6F1 expression in transfected 293T cell line by POU6F1 monoclonal antibody (M01), clone 6H1.Lane 1: POU6F1 transfected lysate(32.6 KDa).Lane 2: Non-transfected lysate.

Western Blot: POU6F1 Antibody (6H1) [H00005463-M01] - Analysis of POU6F1 over-expressed 293 cell line, cotransfected with POU6F1 Validated Chimera RNAi ( Cat # H00005463-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POU6F1 monoclonal antibody (M01), clone 6H1 (Cat # H00005463-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: POU6F1 Antibody (6H1) [H00005463-M01] - POU6F1 monoclonal antibody (M01), clone 6H1 Analysis of POU6F1 expression in Jurkat.

Sandwich ELISA: POU6F1 Antibody (6H1) [H00005463-M01] - Detection limit for recombinant GST tagged POU6F1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 6H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

POU6F1 Antibody (6H1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: POU6F1 (NP_002693, 193 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITPKSAQKLKPVLEKWLNEAELRNQEGQQNLMEFVGGEPSKKRKRRTSFTPQAIEALNAYFEKNPLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKLNVFQIP
• Specificity: POU6F1 - POU domain, class 6, transcription factor 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POU6F1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POU6F1 Antibody (6H1) - Azide and BSA Free

• Brain-specific homeobox/POU domain protein 5
• Brn-5
• BRN5brain-5
• mPOU homeobox protein
• MPOUbrn-5
• POU class 6 homeobox 1
• POU domain, class 6, transcription factor 1
• TCFB1Brain-5

Background

Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') (By similarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU6F1
• Entrez:
  • 5463(Human)
• Uniprot:
  • NP_002693(Human)