Porimin Antibody (CL14381) - Azide and BSA Free

Analysis in human tonsil and skeletal muscle tissues using NBP3-44335 antibody. Corresponding Porimin RNA-seq data are presented for the same tissues.

Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.

• Reactivity: Hu
• Applications: IHC
• Clone: CL14381
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Porimin Antibody (CL14381) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Porimin Antibody (CL14381) - Azide and BSA Free (NBP3-26852) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q8N131, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMEM123
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Porimin Antibody (CL14381) - Azide and BSA Free

• KCT3
• KCT-3
• KCT3keratinocytes associated transmembrane protein 3
• Keratinocytes-associated transmembrane protein 3
• Porimin
• PORIMINporimin
• pro oncosis receptor inducing membrane injury
• Pro-oncosis receptor inducing membrane injury
• serine/threonine-rich receptor
• TMEM123
• transmembrane protein 123PORMIN

Background

Porimin was identified by a monoclonal antibody developed by immunizing mice with apoptotic cells. One of these antibodies, designated anti-Porimin (for pro-oncosis receptor inducing membrane injury), was found to directly induce a unique type of cell death in Jurkat cells by binding to a 110-kDa cell surface receptor on Jurkat cells. Anti-Porimin-mediated cell death was preceded by cell aggregation, formation of plasma membrane pores, and the appearance of membrane blebs. More important, these cells show neither DNA fragmentation nor apoptotic bodies, but display lethal damage of the cell membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEM123