Polycystin-1/PKD1 Antibody (PKS161f/B7) - BSA Free

The effects of downregulation and overexpression of PKD-1 on the phenotypes of aortic VSMCs (n = 6 for each group). (A) PKD-1 knockdown inhibited the expression of (A,B1) PC-1 (p < 0.0001, p < 0.0001), (A,B2) calponin (p = 0.0001, p = 0.0008) and (A,B3) SM22 alpha (p = 0.0053, p = 0.0194) as well as elevated the expression of (A,B4) OPN (p < 0.0001, p = 0.0037), (A,B5) MYH10 (p < 0.0001, p = 0.0001), (A,B7) MMP-2 (p < 0.0001, p = 0.0003) Rapamycin didn’t affect the expression of (A,B1) PC-1 (p < 0.0001, p < 0.0001) in sh-PKD-1 VSMCs but elevated the expression of (A,B2) calciponin (p = 0.0026, p = 0.0038) and (A,B3) SM22 alpha (p = 0.0352, p = 0.034) and downregulated the expression of (A,B4) OPN (p = 0.0009, p = 0.0101), (A,B5) MYH10 (p = 0.0002, p = 0.0105), (A,B7) MMP2 (p < 0.0001, p = 0.0031). (A,B6) Showed the protein expression of MMP-1 among three groups. Treatment of sg-PKD-1 enhanced the expression of (C,D1) PC-1 (p < 0.0001, p < 0.0001), (C,D2) calciponin (p = 0.0054, p = 0.0004) and (C,D3) SM22 alpha (p = 0.0005, p = 0.0003), but inhibited the expression of (C,D4) OPN (p = 0.0125, p = 0.0002), (C,D5) MYH10 (p = 0.0078, p = 0.0002), (C,D7) MMP2 (p < 0.0001, p = 0.0009). (C,D6) Showed the protein expression of MMP-1between normal and sg-PKD-1 VSMCs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (compared with normal VSMCs); ##p < 0.01, ###p < 0.001 (compared with TAD VSMCs). #Means the comparison between sh-PKD1 and sh-PKD1 + Rapa VSMCs. p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33071810), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The effects of downregulation and overexpression of PKD-1 on the phenotypes of aortic VSMCs (n = 6 for each group). (A) PKD-1 knockdown inhibited the expression of (A,B1) PC-1 (p < 0.0001, p < 0.0001), (A,B2) calponin (p = 0.0001, p = 0.0008) and (A,B3) SM22 alpha (p = 0.0053, p = 0.0194) as well as elevated the expression of (A,B4) OPN (p < 0.0001, p = 0.0037), (A,B5) MYH10 (p < 0.0001, p = 0.0001), (A,B7) MMP-2 (p < 0.0001, p = 0.0003) Rapamycin didn’t affect the expression of (A,B1) PC-1 (p < 0.0001, p < 0.0001) in sh-PKD-1 VSMCs but elevated the expression of (A,B2) calciponin (p = 0.0026, p = 0.0038) and (A,B3) SM22 alpha (p = 0.0352, p = 0.034) and downregulated the expression of (A,B4) OPN (p = 0.0009, p = 0.0101), (A,B5) MYH10 (p = 0.0002, p = 0.0105), (A,B7) MMP2 (p < 0.0001, p = 0.0031). (A,B6) Showed the protein expression of MMP-1 among three groups. Treatment of sg-PKD-1 enhanced the expression of (C,D1) PC-1 (p < 0.0001, p < 0.0001), (C,D2) calciponin (p = 0.0054, p = 0.0004) and (C,D3) SM22 alpha (p = 0.0005, p = 0.0003), but inhibited the expression of (C,D4) OPN (p = 0.0125, p = 0.0002), (C,D5) MYH10 (p = 0.0078, p = 0.0002), (C,D7) MMP2 (p < 0.0001, p = 0.0009). (C,D6) Showed the protein expression of MMP-1between normal and sg-PKD-1 VSMCs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (compared with normal VSMCs); ##p < 0.01, ###p < 0.001 (compared with TAD VSMCs). #Means the comparison between sh-PKD1 and sh-PKD1 + Rapa VSMCs. p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33071810), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PKS161f/B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Polycystin-1/PKD1 Antibody (PKS161f/B7) - BSA Free Summary

• Immunogen: Recombinant protein (233 aa)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Polycystin-1/PKD1 Antibody (PKS161f/B7) - BSA Free

• Autosomal dominant polycystic kidney disease 1 protein
• PBP
• Pc-1
• PKD1
• polycystic kidney disease 1 (autosomal dominant)
• polycystic kidney disease-associated protein
• polycystin 1
• Polycystin1
• Polycystin-1
• transient receptor potential cation channel, subfamily P, member 1
• TRPP1

Background

Polycystin-1 (also PKD1) is a 500-550 kDa member of the polycystin family of proteins. It is expressed in renal tubule primary cilia, and the membrane region that forms adherens junctions. Polycystin-1 binds to polycystin-2, promoting its insertion into the cell membrane, and regulating its calcium channel activity. In conjunction with polycystin-2, it detects fluid flow and converts this information into calcium signals. It also exists in the ER, where it negatively modulates polycystin-2 mediated calcium release. Mature human polycystin-1 is a 4280 amino acid (aa), 11 transmembrane glycoprotein. Its N-terminal extracellular region (aa 24-3074) is highly modular, and contains a C-type lecin domain, multiple Leu-rich repeats and PKD domains, one GPS region and a PLAT, REJ, WSC and LDLR domain. The cytoplasmic C-terminus (aa 4107-4303) contains a polycystin-2 coiled-coil interaction domain (aa 4220-4251). A percentage of polycystin-1 undergoes autoproteolysis after Leu3048, generating a presumed N-terminal fragment of ~ 350-400 kDa, and a membrane-bound C-terminal fragment of 150 kDa. Over aa 4141-4303, human polycystin-1 shares 71% aa identity with mouse polycystin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Polycystin-1/PKD1 Antibody (NBP2-50247)(1)

Publication using NBP2-50247

• Zhang J, Liu F, He YB et Al. Polycystin-1 Downregulation Induced Vascular Smooth Muscle Cells Phenotypic Alteration and Extracellular Matrix Remodeling in Thoracic Aortic Dissection Front Physiol 2020-04-29 [PMID: 33071810]

Bioinformatics

• Gene Symbol: PKD1